RNA splicing for 30 years

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RNA Splicing

RNA splicing, a post-transcriptional process necessary to form a mature mRNA, was discovered in the late 1970s. Two different modes of splicing have been defined, that is, constitutive splicing and alternative splicing. Constitutive splicing is the process of removing introns from the premRNA, and joining the exons together to form a mature mRNA. Alternative splicing, on the other hand, is the ...

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RNA splicing

In the 20 years since the discovery that many eukaryotic genes are interrupted by non-coding intervening sequences, or introns, biologists have learnt a tremendous amount about the nuclear machinery that removes these introns from precursor gene transcripts before they are translated into proteins. This nuclear machinery must recognize the introns in the mRNA transcripts and then excise them by...

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Understanding splicing regulation through RNA splicing maps

Alternative splicing is a highly regulated process that greatly increases the proteome diversity and plays an important role in cellular differentiation and disease. Interactions between RNA-binding proteins (RBPs) and pre-mRNA are the principle regulator of splicing decisions. Findings from recent genome-wide studies of protein-RNA interactions have been combined with assays of the global effe...

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Mechanism for RNA splicing of gene transcripts.

It has been demonstrated that structural genes in eukaryotes contain intervening sequences (introns) which are absent from expressed sequences (exons) [l-6]. There is evidence that intervening sequences are transcribed [7], but subsequently the introns are removed by a process which is now known as RNA splicing [ 8,9]. RNA splicing must be a very precise process since it results in the joining ...

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A dual role for RNA splicing signals.

gene expression in eukaryotes requires the excision of introns from pre-mrNas by splicing, which eliminates intervening sequences that interrupt the translation open-reading frame in mrNas. the spliceosome—a complex of small rNas and proteins—recognizes and acts on sequences at the exon–intron junctions—5'-splice site [SS] and 3'-SS—and at the branchpoint (Fig 1). Splicing is traditionally cons...

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ژورنال

عنوان ژورنال: RNA

سال: 2015

ISSN: 1355-8382,1469-9001

DOI: 10.1261/rna.050021.115